Phosphatases
PP2A A Subunit (81G5) Rabbit mAb #2041
| Item # | Description | List Price | Web Price | QTY | Buy |
|---|
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P IF-IC | H M Mk R | Endogenous | 62 | Rabbit |
Applications Key:
W = Western Blotting IHC-P = Immunohistochemistry (Paraffin) IF-IC = Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H = Human M = Mouse Mk = Monkey R = Rat
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
PP2A A Subunit (81G5) Rabbit mAb detects endogenous levels of PP2A A subunit, both α and β isoforms. The antibody does not cross-react with other PP2A subunits.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PP2A A Subunit protein.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using PP2A A Subunit (81G5) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PP2A A Subunit (81G5) Rabbit mAb.
Western Blotting
Western blot analysis of extracts from various cell lines using PP2A A Subunit (81G5) Rabbit mAb.
Background
Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8).
- Janssens, V. and Goris, J. et al. (2001) Biochem. J.353, 417 - 439.
- Zolnierowicz, S. et al. (2000) Biochem. Pharmacol.60, 1225 - 1235.
- Milward, T.A. et al. (1999) Trends Biochem. Sci.24, 186 - 191.
- Chen, J. et al. (1992) Science257, 1261 - 1264.
- Turowski, P. et al. (1995) J. Cell. Biol.129, 397 - 410.
- Lee, J. et al. (1996) Proc. Natl. Acad. Sci. USA93, 6043 - 6047.
- Tolstykh, T. et al. (2000) EMBO J.19, 5682 - 5691.
- Yu, X.X. et al. (2001) Mol. Biol. Cell12, 185 - 199.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4953 PP2A B Subunit Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 2038 PP2A C Subunit Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 1054 PP2A A Subunit Blocking Peptide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
