Ca, cAMP & Lipid Signaling
PLCbeta3 Antibody #2482
| Item # | Description | List Price | Web Price | QTY | Buy |
|---|
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M Mk R | Endogenous | 150 | Rabbit |
Applications Key:
W = Western Blotting
Reactivity Key:
H = Human M = Mouse Mk = Monkey R = Rat
Species cross-reactivity is determined by Western blot.
Protocols
- 2482
- Western Blotting,
Specificity / Sensitivity
PLCbeta3 Antibody detects endogenous levels of total PLCbeta3 protein. The antibody does not cross-react with PLCbeta1, PLCbeta2 or PLCbeta4.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residues surrounding carboxy-terminal residues of human PLCbeta3. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or PMA-treated for the indicated times, using PLCbeta3 Antibody.
Background
Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ and PLCε. The PLCβ subfamily includes four members, PLCβ1-4. All four members of the subfamily are activated by α- or β-γ-subunits of the heterotrimeric G-proteins (2,3).
Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of Ser1105 by PKA or PKC inhibits PLCβ3 activity (4,5). Ser537 of PLCβ3 is phosphorylated by CaMKII, and this phosphorylation may contribute to the basal activity of PLCβ3. PLCγ is activated by both receptor and nonreceptor tyrosine kinases (6).
PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783 and 1245 (7). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (8).
- Singer, W. D. et al. (1997) Annu. Rev. Biochem.66, 475 - 509.
- Smrcka, A. V. et al. (1991) Science251, 804 - 807.
- Taylor, S. J. et al. (1991) Nature350, 516 - 518.
- Yue, C. et al. (1998) J. Biol. Chem.273, 18023 - 18027.
- Yue, C. et al. (2000) J. Biol. Chem.275, 30220 - 30225.
- Margolis, B. et al. (1989) Cell57, 1101 - 1107.
- Kim, H. K. et al. (1991) Cell65, 435 - 441.
- Wang, Z. et al. (1998) Mol. Cell. Biol.18, 590 - 597.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 3874 Phospho-PLCγ2 (Tyr759) Antibody
- 3871 Phospho-PLCγ2 (Tyr1217) Antibody
- 2821 Phospho-PLCγ1 (Tyr783) Antibody
- 2481 Phospho-PLCbeta3 (Ser537) Antibody
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3872 PLCγ2 Antibody
- 2484 Phospho-PLCbeta3 (Ser1105) Antibody
- 2822 PLCγ1 Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
