Neuroscience

CREB (48H2) Rabbit mAb (Biotinylated) #4034

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Applications Key:   W = Western Blotting
Reactivity Key: Dm = D. melanogaster  H = Human  M = Mouse  Mk = Monkey  R = Rat  
Species cross-reactivity is determined by Western blot.

Protocols

4034

Western Blotting,

Specificity / Sensitivity

CREB (48H2) Rabbit mAb (Biotinylated) detects endogenous levels of total CREB protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with GST-CREB full-length fusion protein.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross reactivity as the unconjugated CREB (48H2) Rabbit mAb #9197.

Western Blotting

Western blot analysis of extracts from NIH/3T3 and SK-N-MC cells using CREB (48H2) Rabbit mAb (Biotinylated) and developed using Streptavidin-HRP #3999.

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (7-9).

1. Lonze, B.E. et al. (2002) Neuron34, 371 - 85.
2. Lee, M.M. et al. (1999) J Neurosci Res55, 702 - 12.
3. Redmond, L. et al. (2002) Neuron34, 999 - 1010.
4. Dash, P.K. et al. (1990) Nature345, 718 - 21.
5. Yin, J.C. et al. (1994) Cell79, 49 - 58.
6. Guzowski, J.F. and McGaugh, J.L. et al. (1997) Proc Natl Acad Sci USA94, 2693 - 8.
7. Xing, J. et al. (1998) Mol Cell Biol18, 1946 - 55.
8. Ribar, T.J. et al. (2000) J Neurosci20, - .
9. Tan, Y. et al. (1996) EMBO J15, 4629 - 42.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 9191 Phospho-CREB (Ser133) Antibody
• 7727 Biotinylated Protein Ladder Detection Pack
• 3999 Streptavidin-HRP
• 9187 Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor® 488 Conjugate)
• 9190 PhosphoPlus® CREB (Ser133) Antibody Kit
• 9104 CREB (86B10) Mouse mAb
• 9197 CREB (48H2) Rabbit mAb
• 9198 Phospho-CREB (Ser133) (87G3) Rabbit mAb
• 9196 Phospho-CREB (Ser133) (1B6) Mouse mAb

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.