Apoptosis and Autophagy

Survivin (71G4B7) Rabbit mAb (Biotinylated) #4037

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Applications Key:   W = Western Blotting  F = Flow Cytometry
Reactivity Key: H = Human  M = Mouse  R = Rat  
Species cross-reactivity is determined by Western blot.

Protocols

4037

Western Blotting, Flow,

Specificity / Sensitivity

Survivin (71G4B7) Rabbit mAb (Biotinylated) detects endogenous levels of total survivin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys60 of human survivin protein.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody exhibits the same species cross-reactivity as the unconjugated Survivin (71G4B7) Rabbit mAb #2808.

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells using Survivin (71G4B7) Rabbit mAb (Biotinylated) (blue) compared to Rabbit (DA1E) mAb IgG XP™ Isotype Control (Biotinylated) #4096 (red).

Western Blotting

Western blot analysis of extracts from various cell lines using Survivin (71G4B7) Rabbit mAb (Biotinylated).

Background

Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle through inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, has been shown to inhibit tumor growth (5,6).

1. Reed, J.C. and Reed, S.I. et al. (1999) Nature Cell Biol.1, 199 - 200.
2. Li, F. et al. (1998) Nature396, 580 - 584.
3. Li, F. et al. (1999) Nat. Cell Biol.1, 461 - 466.
4. O'Connor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA97, 13103 - 13107.
5. Olie, R.A. et al. (2000) Cancer Res.60, 2805 - 2809.
6. Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA98, 635 - 640.

Application References

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Companion Products

• 3947 Survivin (71G4B7) Rabbit mAb (Sepharose Bead Conjugate)
• 3999 Streptavidin-HRP
• 2808 Survivin (71G4B7) Rabbit mAb

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.