Lymphocyte Signaling

CD44 (156-3C11) Mouse mAb (Biotinylated) #4041

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Applications Key:   W = Western Blotting  F = Flow Cytometry
Reactivity Key: H = Human  
Species cross-reactivity is determined by Western blot.

Protocols

4041

Western Blotting, Flow,

Specificity / Sensitivity

CD44 (156-3C11) Mouse mAb (Biotinylated) detects endogenous levels of total CD44 protein.

Source / Purification

Monoclonal antibody is produced by immunizing BALB/c mice with stimulated human leukocytes.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody exhibits the same species cross-reactivity as the unconjugated CD44 (156-3C11) Mouse mAb #3570.

Western Blotting

Western blot analysis of extracts from HeLa and PANC-1 cells using CD44 (156-3C11) Mouse mAb (Biotinylated) and developed using Streptavidin-HRP #3999.

Flow Cytometry

Flow cytometric analysis of HeLa cells using CD44 (156-3C11) Mouse mAb (Biotinylated) (blue) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) #4097 (red).

Background

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM). CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1,2). Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. Interactions between CD44 and HER2 have been linked to an increase in ovarian carcinoma cell growth (1-3). CD44 interacts with ezrin, radixin and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM (4-6). CD44 is constitutively phosphorylated at Ser325 in resting cells. Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility (4).

1. Goodison, S. et al. (1999) Mol. Pathol.52, 189 - 196.
2. Cichy, J. and Puré, E. et al. (2003) J. Cell Biol.161, 839 - 843.
3. Bourguignon, L.Y. et al. (1997) J. Biol. Chem.272, 27913 - 27918.
4. Legg, J.W. et al. (2002) Nat. Cell Biol.4, 399 - 407.
5. Yonemura, S. et al. (1998) J. Cell Biol.140, 885 - 895.
6. Tsukita, S. et al. (1994) J. Cell Biol.126, 391 - 401.

Application References

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Companion Products

• 4443 CD3ε (CD3-12) Rat mAb
• 3578 CD44 Antibody
• 3516 CD44 (156-3C11) Mouse mAb (Alexa Fluor® 488 Conjugate)
• 3999 Streptavidin-HRP
• 3565 CD10 (CB-CALLA) Mouse mAb
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 3576 CD56 (NCAM) (123C3) Mouse mAb
• 3563 CD4 (Edu-2) Mouse mAb
• 7727 Biotinylated Protein Ladder Detection Pack
• 3569 CD34 (ICO115) Mouse mAb
• 3572 CD8 (RIV11) Mouse mAb

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.