Translational Control

hnRNP K (A222) Antibody #4699

• » more info
• » application references
• » datasheet PDF
• » MSDS PDF
• » protocols

• » complementary products
• » compare antibodies
• » control extracts

Applications Key:   W = Western Blotting  IP = Immunoprecipitation
Reactivity Key: H = Human  M = Mouse  R = Rat  
Species cross-reactivity is determined by Western blot.

Protocols

4699

Western Blotting, Immunoprecipitation,

Specificity / Sensitivity

hnRNP K (A222) Antibody detects endogenous levels of total hnRNP K protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala222 of human hnRNP K protein. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from HeLa and Hep G2 cells using hnRNP K (A222) Antibody.

Background

Heterogeneous nuclear ribonucleoprotein K (hnRNP K) belongs to a family of RNA binding multiprotein complexes (hnRNP proteins) that facilitate pre-mRNA processing and transport of mRNA from the nucleus to cytoplasm (1-3). hnRNP K contains three unique structural motifs termed KH domains that bind poly(C) DNA and RNA sequences (4,5). Intricate architecture enables hnRNP K to facilitate mRNA biosynthesis (6), transcriptional regulation (7) and signal transduction. Cytoplasmic hnRNP K expression is increased in oral squamous cell carcinoma and pancreatic cancer and may be a potential prognostic factor (8,9). hnRNP K coordinates with p53 to regulate its target gene transcription in response to DNA damage. Proteasome degradation of hnRNP K is mediated by E3 ligase MDM2 (10). The interaction between hnRNP K and c-Src leads to hnRNP K phosphorylation, which allows for hnRNP K activation of silenced mRNA translation (11).

1. Dreyfuss, G. et al. (1993) Annu Rev Biochem62, 289 - 321.
2. Siomi, H. et al. (1994) Cell77, 33 - 9.
3. Miau, L.H. et al. (1998) J Biol Chem273, 10784 - 91.
4. Tomonaga, T. and Levens, D. et al. (1995) J Biol Chem270, 4875 - 81.
5. Choi, H.S. et al. (2009) Biochem Biophys Res Commun380, 431 - 6.
6. Bustelo, X.R. et al. (1995) Mol Cell Biol15, 1324 - 32.
7. Michelotti, E.F. et al. (1996) Mol Cell Biol16, 2350 - 60.
8. Zhou, R. et al. (2010) Int J Cancer126, 395 - 404.
9. Matta, A. et al. (2009) Int J Cancer125, 1398 - 406.
10. Moumen, A. et al. (2005) Cell123, 1065 - 78.
11. Ostareck-Lederer, A. et al. (2002) Mol Cell Biol22, 4535 - 43.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 4675 hnRNP K (R332) Antibody
• 4296 hnRNP A1 (K350) Antibody
• 4783 hnRNP LL Antibody
• 9304 hnRNP A2/B1 (2A2) Mouse mAb
• 4046 hnRNP A0 Antibody
• 5380 hnRNP A1 (R196) Antibody

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.