NF-kappaB Signaling

Phospho-RelB (Ser552) (D41B9) XP™ Rabbit mAb #5025

• » more info
• » application references
• » datasheet PDF
• » MSDS PDF
• » protocols

• » complementary products
• » compare antibodies
• » control extracts

Applications Key:   W = Western Blotting  IP = Immunoprecipitation  IF-IC = Immunofluorescence (Immunocytochemistry)  F = Flow Cytometry
Reactivity Key: B = Bovine  Dg = Dog  H = Human  M = Mouse  Mk = Monkey  R = Rat  
Species cross-reactivity is determined by Western blot.

Protocols

5025

Western Blotting, Flow, Immunofluorescence, Immunoprecipitation,

Specificity / Sensitivity

Phospho-RelB (Ser552) (D41B9) XP™ Rabbit mAb detects endogenous levels of RelB only when phosphorylated at Ser552.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser552 of human RelB protein.

Flow Cytometry

Flow cytometric analysis of Raji cells, untreated (blue) or treated with TPA #4174 (green), using Phospho-RelB (Ser552) (D41B9) XP™ Rabbit mAb.

IF-IC

Immunofluorescent analysis of Raji cells, treated with TPA #4174 (left) or serum-starved (right) using Phospho-RelB (Ser552) (D41B9) XP™ Rabbit mAb (green). Red = Propidium Iodide (fluorescent DNA dye).

Western Blotting

Western blot analysis of extracts from Raji cells, untreated or treated with TPA #4174 (200 nM, 30 minutes) alone or with λ phosphatase plus calf intestinal phosphatase (CIP), using Phospho-RelB (Ser552) (D41B9) XP™ Rabbit mAb (upper) and RelB (C1E4) Rabbit mAb #4922 (lower).

Background

Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50) and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through an ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).

1. Baeuerle, P.A. and Henkel, T. et al. (1994) Annu Rev Immunol12, 141 - 79.
2. Baeuerle, P.A. and Baltimore, D. et al. (1996) Cell87, 13 - 20.
3. Haskill, S. et al. (1991) Cell65, 1281 - 9.
4. Thompson, J.E. et al. (1995) Cell80, 573 - 82.
5. Whiteside, S.T. et al. (1997) EMBO J16, 1413 - 26.
6. Traenckner, E.B. et al. (1995) EMBO J14, 2876 - 83.
7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA92, 11259 - 63.
8. Chen, Z.J. et al. (1996) Cell84, 853 - 62.
9. Senftleben, U. et al. (2001) Science293, 1495 - 9.
10. Coope, H.J. et al. (2002) EMBO J21, 5375 - 85.
11. Xiao, G. et al. (2001) Mol Cell7, 401 - 9.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 4922 RelB (C1E4) Rabbit mAb
• 4999 Phospho-RelB (Ser552) Antibody
• 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7003 20X LumiGLO® Reagent and 20X Peroxide
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.