Translational Control

ERp72 (D70D12) XP™ Rabbit mAb #5033

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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IF-IC F	H M Mk R	Endogenous	72	Rabbit

Applications Key: W = Western Blotting IF-IC = Immunofluorescence (Immunocytochemistry) F = Flow Cytometry
Reactivity Key: H = Human M = Mouse Mk = Monkey R = Rat
Species cross-reactivity is determined by Western blot.

Protocols

5033: Western Blotting, Flow, Immunofluorescence,

Specificity / Sensitivity

ERp72 (D70D12) XP™ Rabbit mAb detects endogenous levels of total ERp72 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the residues surrounding Met279 of human ERp72 protein.

Western Blotting

Western blot analysis of extracts from various cell lines using ERp72 (D70D12) XP™ Rabbit mAb.

IF-IC

Confocal immunofluorescent analysis of PANC-1 cells using ERp72 (D70D12) XP™ Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow cytometric analysis of Hep G2 cells using ERp72 (D70D12) XP™ Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

Background

Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (2). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum stress protein 72 (ERp72), constitute the PDI family (3,4). ERp72 contains three thioredoxin homology domains (3) and plays a role in the formation and isomerization of disulfide bonds on secretory proteins (3,4).

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.