Cytoskeletal Signaling
Pan-Keratin (C11) Mouse mAb (PE Conjugate) #5075
| Item # | Description | List Price | Web Price | QTY | Buy |
|---|
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| F | H Mk R | Endogenous | 46, 55 | Mouse |
Applications Key:
F = Flow Cytometry
Reactivity Key:
H = Human Mk = Monkey R = Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Pan-Keratin (C11) Mouse mAb (PE Conjugate) detects endogenous levels of total keratin 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins.
Source / Purification
Monoclonal antibody is produced by immunizing a BALB/c mouse with a cytoskeleton preparation from A-431 cells.
Description
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Pan-Keratin (C11) Mouse mAb #4545.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using Pan-Keratin (C11) Mouse mAb (PE Conjugate) (blue) compared to a nonspecific negative control antibody (red).
Background
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
- Moll, R. et al. (1982) Cell31, 11 - 24.
- Chang, L. and Goldman, R.D. et al. (2004) Nat Rev Mol Cell Biol5, 601 - 13.
- Ramaekers, F.C. and Bosman, F.T. et al. (2004) J Pathol204, 351 - 4.
- Lane, E.B. and McLean, W.H. et al. (2004) J Pathol204, 355 - 66.
- Zatloukal, K. et al. (2004) J Pathol204, 367 - 76.
- Owens, D.W. and Lane, E.B. et al. (2004) J Pathol204, 377 - 85.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
