Cytoskeletal Signaling

Rab9 (D52G8) XP™ Rabbit mAb #5118

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Applications Key:   W = Western Blotting  IP = Immunoprecipitation  IF-IC = Immunofluorescence (Immunocytochemistry)
Reactivity Key: H = Human  M = Mouse  Mk = Monkey  R = Rat  
Species cross-reactivity is determined by Western blot.

Protocols

5118

Western Blotting, Immunofluorescence, Immunoprecipitation,

Specificity / Sensitivity

Rab9 (D52G8) XP™ Rabbit mAb detects endogenous levels of total Rab9 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human Rab9 protein.

Western Blotting

Western blot analysis of extracts from various cell lines using Rab9 (D52G8) XP™ Rabbit mAb.

IF-IC

Confocal Immunofluorescent analysis of HeLa cells using Rab9 (D52G8) XP™ Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Rab7 and Rab9 are members of the Ras superfamily of small Rab GTPases (1). Both proteins are located in late endosomes, but exert different functions. Rab7 associates with the RIPL effector protein to control membrane trafficking from early to late endosome and to lysosomes (2,3). Rab7 also helps to regulate growth receptor endocytic trafficking and degradation (3,4), and maturation of phagosome and autophagic vacuoles (4-6). Rab9 interacts with its effector proteins p40 and TIP47 (7,8) to promote the MPR (mannose 6-phosphate receptor)-associated lysosomal enzyme transport between late endosomes and the trans Golgi network (9,10).

1. Zerial, M. and McBride, H. et al. (2001) Nat Rev Mol Cell Biol2, 107 - 17.
2. Feng, Y. et al. (1995) J Cell Biol131, 1435 - 52.
3. Méresse, S. et al. (1995) J Cell Sci108 ( Pt 11), 3349 - 58.
4. Ceresa, B.P. and Bahr, S.J. et al. (2006) J Biol Chem281, 1099 - 106.
5. Jäger, S. et al. (2004) J Cell Sci117, 4837 - 48.
6. Méresse, S. et al. (1999) EMBO J18, 4394 - 403.
7. Díaz, E. et al. (1997) J Cell Biol138, 283 - 90.
8. Barbero, P. et al. (2002) J Cell Biol156, 511 - 8.
9. Lombardi, D. et al. (1993) EMBO J12, 677 - 82.
10. Riederer, M.A. et al. (1994) J Cell Biol125, 573 - 82.

Application References

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Companion Products

• 5133 Rab9 (D22A6) Rabbit mAb
• 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 2095 Rab9 Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.