Cell Cycle / Checkpoint Control
Geminin Antibody #5165
| Item # | Description | List Price | Web Price | QTY | Buy |
|---|
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M Mk R | Endogenous | 25 | Rabbit |
Applications Key:
W = Western Blotting IP = Immunoprecipitation
Reactivity Key:
H = Human M = Mouse Mk = Monkey R = Rat
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
Geminin Antibody detects endogenous levels of total geminin protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly80 of human geminin protein. Antibodies are purified using protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell types using Geminin Antibody.
IP
Immunoprecipitation of geminin from 293T cells using Geminin Antibody. Western blot was performed using the same antibody. Lane 1 contains 5% input.
Background
The initiation of DNA replication in mammalian cells is a highly coordinated process that ensures duplication of the genome only once per cell division cycle. Origins of replication are dispersed throughout the genome and their activities are regulated via the sequential binding of pre-replication and replication factors. The origin recognition complex (ORC) is thought to be bound to chromatin throughout the cell cycle (1,2). The pre-replication complex (pre-RC) forms in late mitosis/early G1 phase beginning with the binding of CDT1 and cdc6 to the origin, which allows binding of the heterohexameric MCM2-7 complex. Once this complex is formed, the origin is “licensed” for initiation of DNA replication. In order to ensure that replication occurs only once per cell cycle, geminin binds to and inhibits CDT1 during the S, G2 and M phases. This prevents the recruitment of the MCM complex to the origins of replication, which blocks the premature reformation of the Pre-RC. At the metaphase/anaphase transition, geminin is degraded by the anaphase-promoting complex (APC) allowing for the formation of new pre-RC (3,4).
- Okuno, Y. et al. (2001) EMBO J20, 4263 - 77.
- McNairn, A.J. et al. (2005) Exp Cell Res308, 345 - 56.
- Montanari, M. et al. (2006) Cell Death Differ13, 1052 - 6.
- McGarry, T.J. and Kirschner, M.W. et al. (1998) Cell93, 1043 - 53.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
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- 3387 Cdc6 (C42F7) Rabbit mAb
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 4731 ORC1 (7A7) Rat mAb
- 3619 MCM2 (D7G11) XP™ Rabbit mAb
- 4736 ORC2 (3G6) Rat mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7727 Biotinylated Protein Ladder Detection Pack
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
