Tyrosine Kinases / Adaptors
VEGF Receptor 2 (55B11) Rabbit mAb (Sepharose Bead Conjugate) #5168
| Item # | Description | List Price | Web Price | QTY | Buy |
|---|
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| IP | H M | Endogenous | 210, 230 | Rabbit |
Applications Key:
IP = Immunoprecipitation
Reactivity Key:
H = Human M = Mouse
Species cross-reactivity is determined by Western blot.
Protocols
- 5168
- Immunoprecipitation,
Specificity / Sensitivity
VEGF Receptor 2 (55B11) Rabbit mAb (Sepharose Bead Conjugate) detects endogenous levels of VEGF receptor 2 protein. This antibody does not cross-react with other family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein containing the carboxy-terminal 150 amino acid residues of human VEGF receptor 2 protein.
Description
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. VEGF Receptor 2 (55B11) Rabbit mAb (Sepharose Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated VEGF Receptor 2 (55B11) Rabbit mAb #2479.
IP
Immunoprecipitation of PAEC/CKR cell lysates using VEGF Receptor 2 (55B11) Rabbit mAb (Sepharose Bead Conjugate) and Rabbit (DA1E) mAb IgG XP™ Isotype Control (Sepharose Bead Conjugate) #3423. The western blot was probed using VEGF Receptor 2 (55B11) Rabbit mAb #2479. PAEC/CKR cells overexpress chimeric receptors containing the human CSF-1 extracellular binding domain/mouse VEGF receptor 2 intracellular domains (7).
Background
Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, Grb2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).
- Meyer, M. et al. (1999) EMBO J18, 363 - 74.
- Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun205, 728 - 38.
- Kroll, J. and Waltenberger, J. et al. (1997) J Biol Chem272, 32521 - 7.
- Takahashi, T. et al. (2001) EMBO J20, 2768 - 78.
- Holmqvist, K. et al. (2004) J Biol Chem279, 22267 - 75.
- Karkkainen, M.J. and Petrova, T.V. et al. (2000) Oncogene19, 5598 - 605.
- Rahimi, N. et al. (2000) J Biol Chem275, 16986 - 92.
- Claesson-Welsh, L. et al. (2003) Biochem Soc Trans31, 20 - 4.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
