Translational Control

eIF4G2/p97 (D88B6) XP™ Rabbit mAb #5169

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Item #	Description	List Price	Web Price	QTY	Buy
5169S	eIF4G2/p97 (D88B6) XP™ Rabbit mAb - 100ul	$300.00	$270.00

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP IF-IC F	H M Mk R	Endogenous	97	Rabbit

Applications Key: W = Western Blotting IP = Immunoprecipitation IF-IC = Immunofluorescence (Immunocytochemistry) F = Flow Cytometry
Reactivity Key: H = Human M = Mouse Mk = Monkey R = Rat
Species cross-reactivity is determined by Western blot.

Protocols

5169: Western Blotting, Flow, Immunofluorescence, Immunoprecipitation,

Specificity / Sensitivity

eIF4G2/p97 (D88B6) XP™ Rabbit mAb detects endogenous levels of total eIF4G2/p97 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu450 of human eIF4G2/p97 protein.

Western Blotting

Western blot analysis of extracts from various cell types using eIF4G2/p97 (D88B6) XP™ Rabbit mAb.

IF-IC

Confocal immunofluorescent analysis of HeLa cells using eIF4G2/p97 (D88B6) XP™ Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow cytometric analysis of HeLa cells using eIF4G2/p97 (D88B6) XP™ Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP™ Isotype Control #3900 (red).

Background

The initiation of translation is an important biological event and a variety of factors contribute to this process. Members of the eIF4 translation initiation factor family bind to the 5' m7GTP mRNA cap and unwind the mRNA secondary structure (1,2). The amino-terminal portion of eIF4G physically associates with eIF4E to stimulate the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A and serves as an adaptor molecule in the eIF4 complex (4). Moreover, eIF4G plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5,6). The eIF4G family includes eIF4G1 (eIF4GI), eIF4G2 (p97, DAP5 or NAT1), and eIF4G3 (eIF4GII) (7). These factors share a homologous sequence that provides for interaction with initiation factors eIF3 and eIF4A. Both eIF4G1 and eIF4G3 are involved in cap-dependent translation, while eIF4G2 plays a role in IRES-mediated translation of some genes during cell stress (7,8).

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.