Growth factors/cytokines
Human Interleukin-29 (hIL-29) #5183
| Item # | Description | List Price | Web Price | QTY | Buy |
|---|
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| 31 kDa |
Source / Purification
Recombinant human IL-29 (hIL-29) Gly20-Thr200 (Accession #NP_742152) was expressed in human 293 cells at Cell Signaling Technology.
Western Blotting
Western blot analysis of extracts from RPMI 8226 cells untreated or treated with hIL-29 for 15 minutes, using Phospho-Stat3 (Tyr705) (D3A7) XP™ Rabbit mAb #9145 (upper) or Stat3 Antibody #9132 (lower).
Bioactivity
The bioactivity of recombinant hIL-29 was determined in a virus protection assay. Hep G2 cells were pre-treated with increasing concentrations of hIL-29 for 24 hours. Cells were then innoculated with encephalomyocarditis virus (EMCV) and incubated for an additional 24 hours. Surviving cells were then fixed and stained with crystal violet and the OD595 was determined.
Coomassie Gel
The purity of recombinant hIL-29 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-29 and staining overnight with Coomassie Blue.
Application References
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Companion Products
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
