NF-kappaB Signaling
Phospho-IRF-7 (Ser471/472) Antibody #5184
| Item # | Description | List Price | Web Price | QTY | Buy |
|---|
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M Mk R | Transfected Only | 65 | Rabbit |
Applications Key:
W = Western Blotting
Reactivity Key:
H = Human M = Mouse Mk = Monkey R = Rat
Species cross-reactivity is determined by Western blot.
Protocols
- 5184
- Western Blotting,
Specificity / Sensitivity
Phospho-IRF-7 (Ser471/472) Antibody detects transfected levels of IRF-7 when phosphorylated at Ser471 and 472.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser471/472 of human IRF-7 protein. Antibodies were purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293T cells, mock transfected or transfected with IKKε and/or IRF-7, using Phospho-IRF-7 (Ser471/472) Antibody (upper) or IRF-7 Antibody #4920 (lower).
Background
Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7 and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2).
- Taniguchi, T. et al. (2001) Annu Rev Immunol19, 623 - 55.
- Honda, K. and Taniguchi, T. et al. (2006) Nat Rev Immunol6, 644 - 58.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4920 IRF-7 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
