PI3K / Akt Signaling

NDRG1 Antibody #5196

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Applications Key:   W = Western Blotting  IP = Immunoprecipitation  IHC-P = Immunohistochemistry (Paraffin)
Reactivity Key: H = Human  M = Mouse  Mk = Monkey  R = Rat  
Species cross-reactivity is determined by Western blot.

Protocols

5196

Western Blotting, IHC / Paraffin, Immunoprecipitation,

Specificity / Sensitivity

NDRG1 Antibody detects endogenous levels of total NDRG1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues in the carboxy terminus of human NDRG1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from various cell lines using NDRG1 Antibody.

Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with SignalSilence® NDRG1 siRNA II #6257, using NDRG1 Antibody (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower).

Background

NDRG1 (N-myc downstream-regulated gene 1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG1 family comprised of four members (NDRG1-4) that function in growth, differentiation and cell survival (1-5). NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage (4), hypoxia (5) and elevated levels of nickel and calcium (2). Expression of NDRG1 is elevated in N-myc defective mice and is negatively regulated by N- and c-myc (1,6). During DNA damage NDRG1 is induced in a p53-dependent fashion and is necessary for p53-mediated apoptosis (4,7). NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth and modulating metastasis and angiogenesis (3,4,6,8,9). Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (10,11). NDRG1 is up-regulated during mast cell maturation and its deletion leads to attenuated allergic responses (12). Both NDGR1 and NDGR2 are substrates for SGK1 although the precise physiological role of this phosphorylation is not known (13). NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366. Phosphorylation by SGK1 primes NDRG1 for phosphorylation by GSK3.

1. Shimono, A. et al. (1999) Mech Dev83, 39 - 52.
2. Zhou, D. et al. (1998) Cancer Res58, 2182 - 9.
3. van Belzen, N. et al. (1997) Lab Invest77, 85 - 92.
4. Kurdistani, S.K. et al. (1998) Cancer Res58, 4439 - 44.
5. Park, H. et al. (2000) Biochem Biophys Res Commun276, 321 - 8.
6. Li, J. and Kretzner, L. et al. (2003) Mol Cell Biochem250, 91 - 105.
7. Stein, S. et al. (2004) J Biol Chem279, 48930 - 40.
8. Maruyama, Y. et al. (2006) Cancer Res66, 6233 - 42.
9. Nishio, S. et al. (2008) Cancer Lett264, 36 - 43.
10. Kalaydjieva, L. et al. (2000) Am J Hum Genet67, 47 - 58.
11. Okuda, T. et al. (2004) Mol Cell Biol24, 3949 - 56.
12. Taketomi, Y. et al. (2007) J Immunol178, 7042 - 53.
13. Murray, J.T. et al. (2004) Biochem J384, 477 - 88.

Application References

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Companion Products

• 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 3506 Phospho-NDRG1 (Ser330) Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 3217 Phospho-NDRG1 (Thr346) Antibody
• 7727 Biotinylated Protein Ladder Detection Pack
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 6245 SignalSilence® NDRG1 siRNA I
• 6257 SignalSilence® NDRG1 siRNA II
• 7003 20X LumiGLO® Reagent and 20X Peroxide
• 8112 SignalStain® Antibody Diluent
• 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
• 8101 SignalSlide® Phospho-Akt (Ser473) IHC Controls

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.