Growth factors/cytokines
Human β-Nerve Growth Factor (hβ-NGF) #5221
| Item # | Description | List Price | Web Price | QTY | Buy |
|---|
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| 13 |
Source / Purification
Recombinant human β-NGF (hβ-NGF) Ser122-Ala241 (Accession #NP_002497) was expressed in human 293 cells at Cell Signaling Technology.
Coomassie Gel
The purity of recombinant hβ-NGF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hβ-NGF and staining overnight with Coomassie Blue.
Western Blotting
Western blot analysis of extracts from TF-1 cells untreated or treated with hβ-NGF for 10 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP™ Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).
Bioactivity
The proliferation of TF-1 cells treated with increasing concentrations of hβ-NGF was assessed. After 48 hour treatment with hβ-NGF, cells were incubated with a tetrazolium salt and the OD450-OD650 was determined.
Application References
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Companion Products
- 4695 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb
- 4370 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP™ Rabbit mAb
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
