Protein Stability

UBA2 Antibody #5293

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Applications Key:   W = Western Blotting  IP = Immunoprecipitation
Reactivity Key: H = Human  M = Mouse  Mk = Monkey  R = Rat  
Species cross-reactivity is determined by Western blot.

Protocols

5293

Western Blotting, Immunoprecipitation,

Specificity / Sensitivity

UBA2 Antibody detects endogenous levels of total UBA2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu490 of human UBA2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from various cell lines using UBA2 Antibody.

Background

The process of SUMO conjugation to target proteins is similar to the molecular chain of events observed with ubiquitin (1). SUMO is conjugated to target proteins through the coordinated action of the cellular SUMO conjugation machinery consisting of E1, E2, and E3 enzymes (2). The canonical SUMO E1 activating enzyme is a heterodimer consisting of SAE1 (AOS1) and UBA2 (SAE2) subunits. Mature SUMO is activated by E1 in an ATP-dependent reaction that generates adenylated SUMO, which functions as a high-energy intermediate in the formation of a thioester linkage between SUMO and Cys173 of UBA2 (3,4). SUMO is subsequently transferred from UBA2 to the SUMO E2 conjugating enzyme, UBC9 (5). Recent evidence suggests that redox regulation of UBA2 serves as a physiologic mechanism to modulate the cellular level of sumoylated target proteins (6).

1. Geiss-Friedlander, R. and Melchior, F. et al. (2007) Nat Rev Mol Cell Biol8, 947 - 56.
2. Tatham, M.H. et al. (2003) Biochemistry42, 9959 - 69.
3. Desterro, J.M. et al. (1999) J Biol Chem274, 10618 - 24.
4. Gong, L. et al. (1999) FEBS Lett448, 185 - 9.
5. Desterro, J.M. et al. (1997) FEBS Lett417, 297 - 300.
6. Bossis, G. and Melchior, F. et al. (2006) Mol Cell21, 349 - 57.

Application References

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.