Phosphatases

Phospho-CTDSPL2 (Ser104) Antibody #5294

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Applications Key:   W = Western Blotting  IP = Immunoprecipitation
Reactivity Key: H = Human  Mk = Monkey  
Species cross-reactivity is determined by Western blot.

Protocols

5294

Western Blotting, Immunoprecipitation,

Specificity / Sensitivity

Phospho-CTDSPL2 (Ser104) Antibody detects endogenous levels of CTDSPL2 only when phosphorylated at Ser104.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser104 of human CTDSPL2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from NCI-H1703 cells, untreated or EGF-treated, and HeLa cells, untreated or λ-phosphatase-treated, using Phospho-CTDSPL2 (Ser104) Antibody (upper) and a total CTDSPL2 antibody (lower).

Background

CTD small phosphatase-like protein 2 (CTDSPL2, HSPC129) is a putative RNA-polymerase II carboxy-terminal domain (CTD) phosphatase (1) that belongs to a small subfamily of CTD phosphatases (2). The CTD of RNA polymerase II contains multiple Y-S-P-T-S-P-S repeats that are phosphorylated during the transcription cycle (3,4). In general, CTD phosphatases regulate the reversible CTD phosphorylation state of RNA-polymerase II at several stages of RNA synthesis and during post-transcriptional modification (4-6). CTDSPL2 has several structural and functional similarities to other CTD phosphatases, including FCP1, SCP1, DULLARD and UBLCP1 (1,2).

1. Qian, H. et al. (2007) Mol Cell Biochem303, 183 - 8.
2. Kim, Y. et al. (2007) Proc Natl Acad Sci USA104, 6596 - 601.
3. Corden, J.L. et al. (1985) Proc Natl Acad Sci USA82, 7934 - 8.
4. Ahn, S.H. et al. (2004) Mol Cell13, 67 - 76.
5. Dahmus, M.E. et al. (1996) J Biol Chem271, 19009 - 12.
6. Goodrich, J.A. and Tjian, R. et al. (1994) Cell77, 145 - 56.

Application References

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Companion Products

• 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.