Apoptosis and Autophagy
Atg4B Antibody #5299
| Item # | Description | List Price | Web Price | QTY | Buy |
|---|
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 48 | Rabbit |
Applications Key:
W = Western Blotting
Reactivity Key:
H = Human M = Mouse R = Rat
Species cross-reactivity is determined by Western blot.
Protocols
- 5299
- Western Blotting,
Specificity / Sensitivity
Atg4B Antibody detects endogenous levels of total Atg4B protein. This antibody detects a band at ~27 kDa of unknown origin.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser372 of human Atg4B protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines using Atg4B Antibody.
Western Blotting
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with mouse Atg4B (+), using Atg4B Antibody.
Western Blotting
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Atg4B siRNA I (+), using Atg4B Antibody #5299 (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower). The Atg4B Antibody confirms silencing of Atg4B expression, while the β-Tubulin (9F3) Rabbit mAb is used as a loading control.
Background
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Control of autophagy was largely discovered in yeast and involves proteins encoded by a set of autophagy-related genes (Atg) (1). Formation of autophagic vesicles requires a pair of essential ubiquitin-like conjugation systems, Atg12-Atg5 and Atg8-phosphatidylethanolamine (Atg8-PE), which are widely conserved in eukaryotes (2). Numerous mammalian counterparts to yeast Atg proteins have been described, including three Atg8 proteins (GATE-16, GABARAP, and LC3) and four Atg4 homologues (Atg4A/autophagin-2, Atg4B/autophagin-1, Atg4C/autophagin-3, and Atg4D/autophagin-4) (3-5). The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE and recycle the Atg8 homologue (6).
- Reggiori, F. and Klionsky, D.J. et al. (2002) Eukaryot Cell1, 11 - 21.
- Ohsumi, Y. et al. (2001) Nat Rev Mol Cell Biol2, 211 - 6.
- Kabeya, Y. et al. (2000) EMBO J19, 5720 - 8.
- Kabeya, Y. et al. (2004) J Cell Sci117, 2805 - 12.
- Mariño, G. et al. (2003) J Biol Chem278, 3671 - 8.
- Sou, Y.S. et al. (2008) Mol Biol Cell19, 4762 - 75.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
