Neuroscience

GAD1 Antibody #5305

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Applications Key:   W = Western Blotting
Reactivity Key: H = Human  M = Mouse  R = Rat  
Species cross-reactivity is determined by Western blot.

Protocols

5305

Western Blotting,

Specificity / Sensitivity

GAD1 Antibody detects endogenous levels of total GAD1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro19 of human GAD1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from mouse and rat brain using GAD1 Antibody.

Background

The enzyme glutamate decarboxylase (GAD) is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid (1). GAD1 (GAD67) and GAD2 (GAD65) are expressed in nervous and endocrine systems (2) and are thought to be involved in synaptic transmission (3) and insulin secretion (4), respectively. Autoantibodies against GAD2 may serve as markers for type I diabetes (5). Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2 (6).

1. Kaufman, D.L. et al. (1991) J Neurochem56, 720 - 3.
2. Feldblum, S. et al. (1993) J Neurosci Res34, 689 - 706.
3. Gao, B. and Moore, R.Y. et al. (1996) J Biol Rhythms11, 172 - 9.
4. Rubi, B. et al. (2001) J Biol Chem276, 36391 - 6.
5. Gilliam, L.K. et al. (2004) Clin Exp Immunol138, 337 - 41.
6. Skorstad, G. et al. (2008) Eur J Neurol15, 973 - 80.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 3988 GAD2 Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 9999 Nonfat Dry Milk
• 9998 BSA
• 7003 20X LumiGLO® Reagent and 20X Peroxide
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.