Neuroscience

AMPA Receptor (GluR 2) (D39F2) Rabbit mAb #5306

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Item #	Description	List Price	Web Price	QTY	Buy
5306S	AMPA Receptor (GluR 2) (D39F2) Rabbit mAb - 100ul	$300.00	$270.00

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W	H M R	Endogenous	100	Rabbit

Applications Key: W = Western Blotting
Reactivity Key: H = Human M = Mouse R = Rat
Species cross-reactivity is determined by Western blot.

Protocols

5306: Western Blotting,

Specificity / Sensitivity

AMPA Receptor (GluR 2) (D39F2) Rabbit mAb detects endogenous levels of total GluR 2 protein. The antibody is not predicted to recognize other AMPA receptor subunits (e.g. GluR 1, GluR 3 or GluR 4) based on sequence homology of the antigen.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GluR 2 protein.

Western Blotting

Western blot analysis of extracts from mouse and rat brains using AMPA Receptor (GluR 2) (D39F2) Rabbit mAb.

Background

AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainite- and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the CNS. AMPARs are implicated in synapse formation, stabilization and plasticity (1). AMPARs that lack GluR 2 are permeable to calcium, in contrast to GluR 2 containing AMPARs (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Activity changes of AMPARs are implicated in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke and epilepsy (1).

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.