Neuroscience
GAP43 Antibody #5307
| Item # | Description | List Price | Web Price | QTY | Buy |
|---|
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 38, 43 | Rabbit |
Applications Key:
W = Western Blotting
Reactivity Key:
H = Human M = Mouse R = Rat
Species cross-reactivity is determined by Western blot.
Protocols
- 5307
- Western Blotting,
Specificity / Sensitivity
GAP43 Antibody detects endogenous levels of total GAP protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GAP43 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from mouse and rat brain using GAP43 Antibody.
Background
GAP43 is a nervous system specific growth-associated protein enriched in growth cones and areas of high plasticity (1). Phosphorylation of GAP43 at Ser41 by PKC is regulated by intracellular Ca2+ and affects the ability of GAP43 to bind calmodulin (2,3). GAP43 is integral to growth cone formation, neurite outgrowth, and development of a functional cerebral cortex (4,5). Aberrant GAP43 expression can be seen in patients diagnosed with schizophrenia and Alzheimer disease (6,7).
- Biewenga, J.E. et al. (1996) Acta Biochim Pol43, 327 - 38.
- Dekker, L.V. and Parker, P.J. et al. (1997) J Biol Chem272, 12747 - 53.
- Chapman, E.R. et al. (1991) J Biol Chem266, 207 - 13.
- Aigner, L. and Caroni, P. et al. (1993) J Cell Biol123, 417 - 29.
- Shen, Y. et al. (2008) Cerebellum7, 451 - 66.
- Sower, A.C. et al. (1995) Mol Chem Neuropathol24, 1 - 11.
- de la Monte, S.M. et al. (1995) Am J Pathol147, 934 - 46.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 2261 Phospho-(Ser) PKC Substrate Antibody
- 4830 Calmodulin Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 2056 PKCα Antibody
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9999 Nonfat Dry Milk
- 9998 BSA
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
