Glucose / Energy Metabolism

ASCT2 (V501) Antibody #5345

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Applications Key:   W = Western Blotting  IP = Immunoprecipitation  IF-IC = Immunofluorescence (Immunocytochemistry)  F = Flow Cytometry
Reactivity Key: H = Human  M = Mouse  R = Rat  
Species cross-reactivity is determined by Western blot.

Protocols

5345

Western Blotting, Flow, Immunofluorescence, Immunoprecipitation,

Specificity / Sensitivity

ASCT2 (V501) Antibody detects endogenous levels of total ASCT2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ASCT2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

IF-IC

Confocal immunofluorescent analysis of HT-29 cells using ASCT2 (V501) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western blot analysis of extracts from HT-29 cells, untreated or treated with peptide N-glycosidase F (PNGase F), and untreated SW620 cells, using ASCT2 (V501) Antibody.

Flow Cytometry

Flow cytometric analysis of HT-29 cells using ASCT2 (V501) Antibody (blue) compared to a nonspecific negative control antibody (red).

Background

Sodium-dependent neutral amino acid transporter type 2 (ASCT2 or SLC1A5) is a neutral amino acid transporter that regulates the uptake of essential amino acids in conjunction with the SLC7A5 bilateral transporter (1,2). ASCT2 appears to be the major glutamine transporter in hepatoma cells and is thought to provide essential amino acids needed for tumor growth (3). Additional evidence suggests that ASCT2 plays a role in activating mTORC1 signaling and is required to suppress autophagy (4,5). Cell surface ASCT2 serves as a receptor for several mammalian interference retroviruses associated with cases of infectious immunodeficiency; variation in a small region of an extracellular loop (ECL2) may be responsible for species-specific differences in receptor function (6).

1. Utsunomiya-Tate, N. et al. (1996) J Biol Chem271, 14883 - 90.
2. Bröer, S. et al. (2008) Physiol Rev88, 249 - 86.
3. Bode, B.P. et al. (2002) Am J Physiol Gastrointest Liver Physiol283, G1062 - 73.
4. Fuchs, B.C. et al. (2007) Am J Physiol Cell Physiol293, C55 - 63.
5. Nicklin, P. et al. (2009) Cell136, 521 - 34.
6. Marin, M. et al. (2003) J Virol77, 2936 - 45.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 5100 ASCT2 (G11) Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO® Reagent and 20X Peroxide
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.