MAP Kinase Signaling

SOS1 Antibody #5890

• » more info
• » application references
• » datasheet PDF
• » MSDS PDF
• » protocols

• » complementary products
• » compare antibodies
• » control extracts

Applications Key:   W = Western Blotting  IP = Immunoprecipitation
Reactivity Key: H = Human  M = Mouse  
Species cross-reactivity is determined by Western blot.

Protocols

5890

Western Blotting, Immunoprecipitation,

Specificity / Sensitivity

SOS1 Antibody detects endogenous levels of total SOS1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile1198 of human SOS1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from various cell lines using SOS1 Antibody.

Background

Son of sevenless (SOS) was first identified in Drosophila as a guanine nucleotide exchange factor (GEF) for Ras acting downstream of the Sevenless receptor (1). Two closely related homologs of Drosophila SOS are found in mammalian cells: SOS1 and SOS2 (2). SOS1 consists of histone folds, Dbl (DH) and pleckstrin (PH) homology domains, a Ras exchange motif (REM), and Cdc25 homology and polyproline domains (3). SOS1 binds to GRB2, NCK and other adaptor proteins, and plays an important role in ERK activation downstream of protein tyrosine kinase receptor (RTK). Mutations in the corresponding SOS1 gene have been found in people with Noonan syndrome, a developmental disorder characterized by short stature, facial dysmorphia and congenital heart defects (4,5).

1. Rogge, R.D. et al. (1991) Cell64, 39 - 48.
2. Nimnual, A. and Bar-Sagi, D. et al. (2002) Sci STKE2002, - .
3. Chardin, P. et al. (1993) Science260, 1338 - 43.
4. Tartaglia, M. et al. (2007) Nat Genet39, 75 - 9.
5. Roberts, A.E. et al. (2007) Nat Genet39, 70 - 4.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 4370 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP™ Rabbit mAb
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 3972 GRB2 Antibody
• 9422 c-Raf Antibody
• 9146 MEK1 (30C8) Rabbit mAb
• 4695 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb
• 7727 Biotinylated Protein Ladder Detection Pack
• 9102 p44/42 MAPK (Erk1/2) Antibody
• 3339 Ras (27H5) Rabbit mAb

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.