Cytoskeletal Signaling
COX IV (3E11) Rabbit mAb (Biotinylated) #6014
| Item # | Description | List Price | Web Price | QTY | Buy |
|---|
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | B H Mk Pg R Z | Endogenous | 17 | Rabbit |
Applications Key:
W = Western Blotting
Reactivity Key:
B = Bovine H = Human Mk = Monkey Pg = Pig R = Rat Z = Zebrafish
Species cross-reactivity is determined by Western blot.
Protocols
- 6014
- Western Blotting,
Specificity / Sensitivity
COX IV (3E11) Rabbit mAb (Biotinylated) detects endogenous levels of total COX IV protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV protein.
Description
This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated COX IV (3E11) Rabbit mAb #4850.
Western Blotting
Western blot analysis of extracts from HeLa, Jurkat and COS cells using COX IV (3E11) Rabbit mAb (Biotinylated) and developed using Streptavidin-HRP #3999.
Background
Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA while the other smaller subunits, including COX IV, are nuclear-encoded. Deficiency in COX activity has been correlated with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control.
- Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol.6, 460 - 466.
- Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta726, 135 - 148.
- Kadenbach, B. et al. (2000) Free Radic. Biol. Med.29, 211 - 221.
- Barrientos, A. et al. (2002) Gene286, 53 - 63.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3999 Streptavidin-HRP
- 5247 COX IV (3E11) Rabbit mAb (HRP Conjugate)
- 4844 COX IV Antibody
- 4850 COX IV (3E11) Rabbit mAb
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
