Cytoskeletal Signaling

β-Tubulin (9F3) Rabbit mAb (Biotinylated) #6181

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Item #	Description	List Price	Web Price	QTY	Buy
6181S	β-Tubulin (9F3) Rabbit mAb (Biotinylated) - 100ul	$399.00	$359.10

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W	B Dm H M Mk R Z	Endogenous	55	Rabbit

Applications Key: W = Western Blotting
Reactivity Key: B = Bovine Dm = D. melanogaster H = Human M = Mouse Mk = Monkey R = Rat Z = Zebrafish
Species cross-reactivity is determined by Western blot.

Protocols

6181: Western Blotting,

Specificity / Sensitivity

β-Tubulin (9F3) Rabbit mAb (Biotinylated) detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human β-tubulin.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated β-Tubulin (9F3) Rabbit mAb #2128.

Western Blotting

Western blot analysis of extracts from NIH/3T3, HeLa and PC-12 cells, using β-Tubulin (9F3) Rabbit mAb (Biotinylated) and detected using Streptavidin-HRP #3999.

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is necessary to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

Westermann, S. and Weber, K. et al. (2003) Nat. Rev. Mol. Cell Biol.4, 938 - 947.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.