SignalSilence siRNA
SignalSilence® MKK3 siRNA I #6294
| Item # | Description | List Price | Web Price | QTY | Buy |
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| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
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| H |
Applications Key:
Reactivity Key:
H = Human
Species cross-reactivity is determined by Western blot.
Description
SignalSilence® MKK3 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit MKK3 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Western Blotting
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® MKK3 siRNA I (+) or SignalSilence® MKK3 siRNA II #6295 (+), using MKK3 Antibody #9232 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The MKK3 Antibody confirms silencing of MKK3 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.
Directions for Use
CST recommends transfection with 100 nM MKK3 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
Background
MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site Thr-Gly-Tyr but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation of serine and threonine residues at sites Ser189 and Thr193 for MKK3 (2) and Ser207 and Thr211 for MKK6 (4,5).
- Derijard, B. et al. (1995) Science267, 682 - 685.
- Raingeaud, J. et al. (1995) J. Biol. Chem.270, 7420 - 7426.
- Sluss, H.K. et al. (1994) Mol. Cell. Biol.14, 8376 - 8384.
- Raingeaud, J. et al. (1996) Mol. Cell. Biol.16(3), 1247 - 1255.
- Han, J. et al. (1996) J. Biol. Chem.271, 2886 - 2891.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 6568 SignalSilence® Control siRNA (Unconjugated)
- 6201 SignalSilence® Control siRNA (Fluorescein Conjugate)
- 6295 SignalSilence® MKK3 siRNA II
- 9232 MKK3 Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
