SignalSilence siRNA
SignalSilence® ABIN-1 siRNA I #6603
| Item # | Description | List Price | Web Price | QTY | Buy |
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| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
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| H |
Applications Key:
Reactivity Key:
H = Human
Species cross-reactivity is determined by Western blot.
Description
SignalSilence® ABIN-1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit ABIN-1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Western Blotting
Western blot analysis of extracts from OVCAR8 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® ABIN-1 siRNA I (+) or SignalSilence® ABIN-1 siRNA II #6609 (+), using ABIN-1 Antibody #4664 (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower). The ABIN-1 Antibody confirms silencing of ABIN-1 expression, while the β-Tubulin (9F3) Rabbit mAb is used as a loading control.
Directions for Use
CST recommends transfection with 100 nM ABIN-1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
Background
The ABIN family (ABIN-1, -2, and -3) is a group of adaptor proteins that associate and cooperate with A20/TNFAIP3 (1), a ubiquitin editing protein that inhibits the key inflammatory transcription factor NF-κB (2-4). Mechanistically, A20 acts by regulating the ubiquitination of the kinase RIP, which leads to inhibition of the IKK complex (5).
- Verstrepen, L. et al. (2009) Biochem Pharmacol78, 105 - 14.
- Beyaert, R. et al. (2000) Biochem Pharmacol60, 1143 - 51.
- Lee, E.G. et al. (2000) Science289, 2350 - 4.
- Dixit, V.M. et al. (1990) J Biol Chem265, 2973 - 8.
- Wertz, I.E. et al. (2004) Nature430, 694 - 9.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4664 ABIN-1 Antibody
- 6609 SignalSilence® ABIN-1 siRNA II
- 6201 SignalSilence® Control siRNA (Fluorescein Conjugate)
- 6568 SignalSilence® Control siRNA (Unconjugated)
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
