SignalSilence siRNA
SignalSilence® Atg7 siRNA I #6604
| Item # | Description | List Price | Web Price | QTY | Buy |
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| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
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| H |
Applications Key:
Reactivity Key:
H = Human
Species cross-reactivity is determined by Western blot.
Description
SignalSilence® Atg7 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Atg7 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Western Blotting
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Atg7 siRNA I (+), using Atg7 Antibody #2631 (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower). The Atg7 Antibody confirms silencing of Atg7 expression, while the β-Tubulin (9F3) Rabbit mAb is used as a loading control.
Directions for Use
CST recommends transfection with 100 nM Atg7 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
Background
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves an ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin-E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).
- Reggiori, F. and Klionsky, D.J. et al. (2002) Eukaryot Cell1, 11 - 21.
- Codogno, P. and Meijer, A.J. et al. (2005) Cell Death Differ12 Suppl 2, 1509 - 18.
- Levine, B. and Yuan, J. et al. (2005) J Clin Invest115, 2679 - 88.
- Mizushima, N. et al. (1998) J Biol Chem273, 33889 - 92.
- Mizushima, N. et al. (1998) Nature395, 395 - 8.
- Suzuki, K. et al. (2001) EMBO J20, 5971 - 81.
- Tanida, I. et al. (1999) Mol Biol Cell10, 1367 - 79.
- Shintani, T. et al. (1999) EMBO J18, 5234 - 41.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 6568 SignalSilence® Control siRNA (Unconjugated)
- 6201 SignalSilence® Control siRNA (Fluorescein Conjugate)
- 2631 Atg7 Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
