Growth factors/cytokines

Human Insulin-like Growth Factor I (hIGF-I) #8917

Item #	Description	List Price	Web Price
8917LF	Human Insulin-like Growth Factor I (hIGF-I) - 250ug	$714.00	$642.60
8917LC	Human Insulin-like Growth Factor I (hIGF-I) - 250ug	$714.00	$642.60
8917SC	Human Insulin-like Growth Factor I (hIGF-I) - 50ug	$238.00	$214.20
8917SF	Human Insulin-like Growth Factor I (hIGF-I) - 50ug	$238.00	$214.20

Applications	Reactivity	Sensitivity	MW (kDa)	Source
			6 kDa

Source / Purification

Recombinant human IGF-I (hIGF-I) Gly49-Ala118 (Accession #P01343) was produced in E. coli at Cell Signaling Technology.

Coomassie Gel

The purity of recombinant hIGF-I was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIGF-I and staining overnight with Coomassie Blue.

Western Blotting

Western blot analysis of extracts from human dermal fibroblasts untreated or treated with hIGF-I for 10 minutes, using Phospho-Akt (Ser473) (D9E) XP™ Rabbit mAb #4060 (upper) and Akt1 (C73H10) Rabbit mAb #2938 (lower).

Western Blotting

Western blot analysis of extracts from MCF-7 cells, untreated or treated with hIGF-I for 10 minutes, using Phospho-Akt (Ser473) (D9E) XP™ Rabbit mAb #4060 (upper) and Akt1 (C73H10) Rabbit mAb #2938 (lower).

Bioactivity

The proliferation of primary human dermal fibroblasts treated with increasing concentrations of hIGF-I was assessed. After 72-hour treatment with hIGF-I cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.