NF-kappaB Signaling
NF-κB Control Cell Extracts #9243
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Description
Untreated NF-κB Control Cell Extracts:
Total cell extracts from HeLa cells prepared without treatment serve as a negative control. Supplied in blue SDS Sample Buffer.
TNF-treated NF-κB Control Cell Extracts:
Total cell extracts from HeLa cells prepared with Human Tumor Necrosis Factor-α (hTNF-α) #8902 treatment serve as a positive control. Supplied in red SDS Sample Buffer.
Western Blotting
Western blot analysis of NF-κB Control Cell Extracts #9243, using IκBα (44D4) Rabbit mAb #4812 (left) and Phospho-IκBα (Ser32) (14D4) Rabbit mAb #2859 (right).
Western Blotting
Western blot analysis of NF-κB Control Cell Extracts #9243, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (left) and NF-κB p65 (E498) Antibody #3987 (right).
Western Blotting
Western blot analysis of NF-κB Control Cell Extracts #9243, using IKKα Antibody #2682 (upper), IKKβ (2C8) Rabbit mAb #2370 (middle) and Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb #2697 (lower).
Applications
Western Blots: CST recommends using 20 µl of untreated and TNF-treated NF-κB cell extracts as controls.
Background
Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50) and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through an ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).
- Baeuerle, P.A. and Henkel, T. et al. (1994) Annu Rev Immunol12, 141 - 79.
- Baeuerle, P.A. and Baltimore, D. et al. (1996) Cell87, 13 - 20.
- Haskill, S. et al. (1991) Cell65, 1281 - 9.
- Thompson, J.E. et al. (1995) Cell80, 573 - 82.
- Whiteside, S.T. et al. (1997) EMBO J16, 1413 - 26.
- Traenckner, E.B. et al. (1995) EMBO J14, 2876 - 83.
- Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA92, 11259 - 63.
- Chen, Z.J. et al. (1996) Cell84, 853 - 62.
- Senftleben, U. et al. (2001) Science293, 1495 - 9.
- Coope, H.J. et al. (2002) EMBO J21, 5375 - 85.
- Xiao, G. et al. (2001) Mol Cell7, 401 - 9.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2697 Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb
- 3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
- 2370 IKKβ (2C8) Rabbit mAb
- 3034 NF-κB p65 Antibody
- 2682 IKKα Antibody
- 4727 c-Rel Antibody
- 4882 NF-κB2 p100/p52 Antibody
- 3035 NF-κB1 p105/p50 Antibody
- 2859 Phospho-IκBα (Ser32) (14D4) Rabbit mAb
- 3037 Phospho-NF-κB p65 (Ser276) Antibody
- 4812 IκBα (44D4) Rabbit mAb
- 4954 RelB Antibody
- 4806 Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 3987 NF-κB p65 (E498) Antibody
- 8902 Human Tumor Necrosis Factor-α (hTNF-α)
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
