Adhesion

β-Catenin Antibody (Amino-terminal Antigen) #9581

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Applications Key:   W = Western Blotting  IP = Immunoprecipitation  IF-F = Immunofluorescence (Frozen)
Reactivity Key: H = Human  M = Mouse  Mk = Monkey  R = Rat  
Species cross-reactivity is determined by Western blot.

Protocols

9581

Western Blotting, Immunofluorescence, Immunoprecipitation,

Specificity / Sensitivity

Beta-Catenin Antibody detects endogenous levels of total β-catenin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp56 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of total cell extracts from 293 and NIH/3T3 cells, using β-Catenin Antibody (Amino-terminal Antigen).

IF-F

Confocal immunofluorescence image of mouse colon labeled with β-Catenin Antibody (Amino-terminal Antigen) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin on Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37 and Thr41 (7). Mutations in these phosphorylation sites, which result in the stabilization of β-catenin protein levels, have been found in many tumor cell lines (8).

1. Cadigan, K.M. and Nusse, R. et al. (1997) Genes Dev.11, 3286 - 3305.
2. Wodarz, A. and Nusse, R. et al. (1998) Annu. Rev. Cell. Dev. Biol.14, 59 - 88.
3. Polakis, P. et al. (1999) Curr. Opin. Genet. Dev.9, 15 - 21.
4. Amit, S. et al. (2002) Genes Dev.16, 1066 - 1076.
5. Lin, C. et al. (2002) Cell108, 837 - 847.
6. Yanagawa, S. et al. (2002) EMBO J.21, 1733 - 1742.
7. Yost, C. et al. (1996) Genes Dev.10, 1443 - 1454.
8. Morin, P.J. et al. (1997) Science275, 1787 - 1790.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 9565 Phospho-β-Catenin (Thr41/Ser45) Antibody
• 9587 β-Catenin Antibody (Carboxy-terminal Antigen)
• 3218 Dvl3 Antibody
• 3216 Dvl2 Antibody
• 9561 Phospho-β-Catenin (Ser33/37/Thr41) Antibody
• 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO® Reagent and 20X Peroxide
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.