Apoptosis and Autophagy
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Biotinylated) #9654
| Item # | Description | List Price | Web Price | QTY | Buy |
|---|
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M Mk R | Endogenous | 17, 19 | Rabbit |
Applications Key:
W = Western Blotting
Reactivity Key:
H = Human M = Mouse Mk = Monkey R = Rat
Species cross-reactivity is determined by Western blot.
Protocols
- 9654
- Western Blotting,
Specificity / Sensitivity
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Biotinylated) detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full-length caspase-3 or other cleaved caspases.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3.
Description
This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664.
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or treated with Staurosporine #9953 (1 μM, 3 hrs), using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Biotinylated) and detected with Streptavidin-HRP #3999.
Background
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).
- Fernandes-Alnemri, T. et al. (1994) J. Biol. Chem.269, 30761 - 30764.
- Nicholson, D. W. et al. (1995) Nature376, 37 - 43.
Application References
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Companion Products
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
