High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one of the most accurate thermostable polymerase available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.
Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl2. All that is required is the addition of template, primers and water.
An E. coli strain that carries the Phusion DNA Polymerase gene.
The following reagents are supplied with this product:
Store at (°C)
Advantages and Features
Robust Reactions - Maximal success with minimal optimization.
Extreme Fidelity - > 50x greater than Taq
High Speed - Dramatically reduced extension times (10x faster than Pfu)
High Yield - Increased product yield using minimal amount of enzyme.
Versatile - Can be used for routine per as well as long or difficult templates.
Long or Difficult Amplification
Properties and Usage
Unit Assay Conditions
25 mM TAPS-HCl (pH 9.3 @ 25°C), 50 mM KCl, 2 mM MgCl2, 1 mM β-mercaptoethanol, 200 µM dNTPs including [3H]-dTTP and 400 µg/ml activated Calf Thymus DNA.
Quality Control Assays
The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
PCR Amplification (Master Mix): The polymerase is tested in a polymerase chain reaction (PCR) using a control template and specific primers, resulting in the expected product.
Products specifications for individual components in the Phusion High-Fidelity PCR Master Mix with HF Buffer are available separately.
Frey, B. and Suppman, B. (1995). Biochemica. 2, 34-35.
Chester, N. and Marshak, D.R. (1993). Analytical Biochemistry. 209, 284-290.
Legal and Disclaimers
Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part
of Thermo Fisher Scientific. This product is manufactured by New England
Biolabs, Inc. under agreement with, and under the performance
specifications of Thermo Fisher Scientific.
Notice to purchaser: Limited license.
The purchase price of this product includes a limited, non-transferable
license under U.S. and foreign patents owned by BIO-RAD Laboratories,
Inc., to use this product. No other license under these patents is
conveyed expressly or by implication by purchase of this product.
PHUSION® is a registered trademark and property of Thermo Fisher Scientific.
THERMO SCIENTIFIC® is a registered trademark and property of Thermo Fisher Scientific.
Material Safety Datasheets
The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at email@example.com.
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at firstname.lastname@example.org or fill out the Technical Support Form for appropriate document.