This Tm Calculator is intended for use in estimating the optimal annealing temperature in PCR reactions. Tm values are calculated using thermodynamic data from Santa Lucia (1998) and the salt correction outlined in Owczarzy et al. (2004). For Phusion DNA polymerases, the thermodynamic data is from Breslauer et al. (1986). This calculator is intended for estimating annealing temperatures for PCR reactions incorporating NEB polymerases.