Survivin Blocking Peptide #1037
Description
This peptide is used to specifically block Survivin (71G4) Rabbit mAb #2808 reactivity.
Quality Control
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
The peptide blocks Survivin (71G4) Rabbit mAb #2808 by immunohistochemistry.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Survivin
(71G4) Rabbit mAb #2808 in the presence of control peptide (left) or Survivin Blocking
Peptide (right).
Applications
Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry
protocols.
Directions For Use
For immunohistochemistry, add twice the volume of peptide as volume of antibody used
in 100 µl total volume. Incubate for a minimum of 30 minutes prior to adding the entire
volume to the slide. Recommended antibody dilutions can be found on the relevant product
data sheet.
Background
Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development
and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling
the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting
cell division (2,3). This regulatory process requires the phosphorylation of survivin
at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective
survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth
(5,6).
- Reed, J.C. and Reed, S.I. et al. (1999) Nature Cell Biol. 1, 199-200.
- Li, F. et al. (1998) Nature 396, 580-584.
- Li, F. et al. (1999) Nat. Cell Biol. 1, 461-466.
- O'Connor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 13103-13107.
- Olie, R.A. et al. (2000) Cancer Res. 60, 2805-2809.
- Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 635-640.
Application References
Have you published research involving the use of our products? If so we'd love to hear about
it. Please let us know!
Companion Products
This product is intended for research purposes only. The product is not intended to
be used for therapeutic or diagnostic purposes in humans or animals.
