New England Biolabs Canada
 

Product Pathways - Protein Folding

Hip Antibody #2723

Item# Description List Price Web Price Qty
2723S Hip Antibody - 100 µl $383.00
$344.70
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human, Mouse, Rat, Monkey Endogenous 48 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting

Protocols

Specificity / Sensitivity

Hip Antibody detects endogenous levels of total Hip protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human Hip. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Hip Antibody.

Background

Hip (HSP70-interacting protein), also known as ST13 (suppression of tumorigenicity protein 13), is one of several co-chaperones that regulate activities of the HSP70 chaperone family (1,2). The homo-oligomeric protein Hip cooperates with HSP70 in protein folding by stabilizing the ADP-bound state of HSP70. Hip directly binds to the ATPase domain of HSP70 when it is converted to the ADP-bound state by proteins of the HSP40 family (3). By collaborating with other positive co-factors such as HSP40 and Hop, or competing with negative co-factors such as Bag1, Hip may facilitate the chaperone function of HSP70 in protein folding and repair, and in controlling the activity of regulatory proteins such as steroid receptors and various regulators of proliferation or apoptosis (4-8).

  1. Prapapanich, V. et al. (1996) Mol. Endocrino.l 10, 420-431.
  2. Gebauer, M. et al. (1997) FEBS Lett. 417, 109-113.
  3. Höhfeld, J. et al. (1995) Cell 83, 589-.98.
  4. Frydman, J. and Höhfeld, J. (1997) Trends Biochem. Sci. 22, 87-92.
  5. Nollen, E.A. et al. (2001) J. Biol. Chem. 276, 4677-4682.
  6. Fan, G.H. et al. (2002) J. Biol. Chem. 277, 6590-6597.
  7. Nelson, G.M. et al. (2004) Mol. Endocrinol. 18, 1620-1630.
  8. Shi, Z.Z. et al. (2007) J. Zhejiang Univ. Sci. B. 8, 170-176.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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