New England Biolabs Canada
 

Product Pathways - Companion Products

Anti-rabbit IgG, HRP-linked Antibody #7074

Item# Description List Price Web Price Qty
7074P2 Anti-rabbit IgG, HRP-linked Antibody - 100 µl $109.00
$98.10
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7074S Anti-rabbit IgG, HRP-linked Antibody - 1 ml $203.00
$182.70
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7074V Anti-rabbit IgG, HRP-linked Antibody - 5 ml $646.00
$581.40
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Protocols

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Description

Designed for use with rabbit polyclonal and monoclonal antibodies, this affinity purified goat anti-rabbit IgG (heavy and light chain) antibody is conjugated to horseradish peroxidase(HRP) for chemiluminescent detection.  This product is thoroughly validated with CST primary antibodies and will work optimally with the CST western immunoblotting protocol, ensuring accurate and reproducible results.

Background

Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

Application References

  • Qattan, A.T. et al. (2010) J Proteome Res 9, 495-508. Applications: Western Blotting.
  • Wu, M. et al. (2010) Rejuvenation Res 13, 571-9. Applications: Western Blotting.
  • Dixon, A.S. et al. (2011) J Biol Chem 286, 27751-60. Applications: Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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