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Product Pathways - PI3K / Akt Signaling

Phospho-PTEN (Ser380) Antibody #9551

Item# Description List Price Web Price Qty
9551S Phospho-PTEN (Ser380) Antibody - 100 µl $446.00
$401.40
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9551T Phospho-PTEN (Ser380) Antibody - 20 µl $180.00
$162.00
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

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12630P SignalFire™ Plus ECL Reagent - 20 ml $120.00
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12630S SignalFire™ Plus ECL Reagent - 100 ml $519.00
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12757P SignalFire™ Elite ECL Reagent - 20 ml $127.00
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12757S SignalFire™ Elite ECL Reagent - 100 ml $558.00
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5384S PTEN (D5G7) Rabbit mAb - 100 µl $383.00
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6883P3 SignalFire™ ECL Reagent - 50 ml $44.00
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6883S SignalFire™ ECL Reagent - 500 ml $382.00
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7074P2 Anti-rabbit IgG, HRP-linked Antibody - 100 µl $112.00
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7074S Anti-rabbit IgG, HRP-linked Antibody - 1 ml $209.00
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7074V Anti-rabbit IgG, HRP-linked Antibody - 5 ml $672.00
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7727L Biotinylated Protein Ladder Detection Pack - 3250 µl $336.00
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7727S Biotinylated Protein Ladder Detection Pack - 650 µl $94.00
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9188L PTEN (D4.3) XP® Rabbit mAb - 300 µl $1,024.00
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9188S PTEN (D4.3) XP® Rabbit mAb - 100 µl $432.00
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9188T PTEN (D4.3) XP® Rabbit mAb - 20 µl $180.00
$162.00
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9549L Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb - 300 µl $1,057.00
$951.30
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9549S Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb - 100 µl $446.00
$401.40
ADD TO CART
9549T Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb - 20 µl $180.00
$162.00
ADD TO CART
9552S PTEN Antibody - 100 µl $383.00
$344.70
ADD TO CART
9554S Phospho-PTEN (Ser380/Thr382/383) Antibody - 100 µl $446.00
$401.40
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9556S PTEN (26H9) Mouse mAb - 100 µl $383.00
$344.70
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9559L PTEN (138G6) Rabbit mAb - 300 µl $938.00
$844.20
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9559S PTEN (138G6) Rabbit mAb - 100 µl $383.00
$344.70
ADD TO CART
9559T PTEN (138G6) Rabbit mAb - 20 µl $163.00
$146.70
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human, Mouse, Rat Endogenous 54 Rabbit
IP

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Homology

Species predicted to react based on 100% sequence homology: Chicken.

Specificity / Sensitivity

Phospho-PTEN (Ser380) Antibody detects endogenous levels of PTEN only when phosphorylated at Ser380.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser380 of human PTEN. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using Phospho-PTEN (Ser380) Antibody (upper) or PTEN Antibody #9552 (lower). The phospho-specificity of the antibody was confirmed by treating the membrane with calf intestinal alkaline phosphatase (CIP) after Western transfer.

Background

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).

  1. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
  2. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.
  3. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
  4. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11.
  5. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.
  6. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.
  7. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.
  8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.
  9. Funamoto, S. et al. (2002) Cell 109, 611-23.
  10. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.

Application References

  • Birle, D. et al. (2002) . J. Immunol. 169, 286-291. Applications: Western Blotting.
  • Sawitzky, M. et al. (2012) PLoS One 7, e39711. Applications: Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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