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Product Pathways - PI3K / Akt Signaling

PTEN (138G6) Rabbit mAb #9559

Item# Description List Price Web Price Qty
9559L PTEN (138G6) Rabbit mAb - 300 µl $938.00
$844.20
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9559S PTEN (138G6) Rabbit mAb - 100 µl $383.00
$344.70
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9559T PTEN (138G6) Rabbit mAb - 20 µl $163.00
$146.70
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat, Monkey Endogenous 54 Rabbit IgG
IP
IHC-P

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)

Specificity / Sensitivity

PTEN (138G6) Rabbit mAb detects endogenous levels of total PTEN protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of human PTEN.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® PTEN siRNA I (+), using PTEN Antibody #9552 and p42 MAPK (Erk2) Antibody #9108. PTEN Antibody confirms silencing of PTEN expression, while the p42 MAPK (Erk2) Antibody is used to control for loading and specificity of PTEN siRNA.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® PTEN siRNA II (+), using PTEN (138G6) Rabbit mAb and β-Actin (13E5) Rabbit mAb #4970. PTEN (138G6) Rabbit mAb confirms silencing of PTEN expression, while the β-Actin (13E5) Rabbit mAb is used to control for loading and specificity of PTEN siRNA.

Western Blotting

Western Blotting

Western blot analysis of extracts from A431, HeLa, 293, COS, PC12, NIH/3T3 and Mouse Brain cells, using PTEN (138G6) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using Phospho-Akt (Ser473) (736E11) Rabbit mAb (IHC Preferred) (#3787) (left) or PTEN (138G6) Rabbit mAb (right). MDA-MB-468 cells lack PTEN. Note the lack of PTEN staining in the Phospho-Akt positive cells.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma (left) and prostate carcinoma (right), using PTEN (138G6) Rabbit mAb. Note the stromal cell staining in the PTEN negative lung carcinoma, and the cancer cell staining in the PTEN positive prostate carcinoma.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PTEN (138G6) Rabbit mAb in the presence of control peptide (left) or PTEN Blocking Peptide #1250 (right).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded cell pellets demonstrating the specificity of PTEN (138G6) Rabbit mAb: DU145, HT-29 and MCF-7 (PTEN positive) and Jurkat, MDA-MB-468 and LNCaP (PTEN negative).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded xenografts using PTEN (138G6) Rabbit mAb. DU145 (left) and A549 (middle) are PTEN positive cell lines, while U-87MG (right) is PTEN negative.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060 (left) or PTEN (138G6) Rabbit mAb (right). Note the presence of P-Akt staining in the PTEN deficient MDA-MB-468 cells.


Background

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).

  1. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
  2. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.
  3. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
  4. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11.
  5. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.
  6. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.
  7. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.
  8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.
  9. Funamoto, S. et al. (2002) Cell 109, 611-23.
  10. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.

Application References

  • Saal, L.H. et al. (2008) Nat Genet 40, 102-7. Applications: IHC-P (paraffin).
  • Laguë, M.N. et al. (2008) Carcinogenesis 29, 2062-72. Applications: IHC-P (paraffin).
  • Guertin, D.A. et al. (2009) Cancer Cell 15, 148-59. Applications: Western Blotting.
  • Sangale, Z. et al. (2011) Appl Immunohistochem Mol Morphol 19, 173-83. Applications: IHC-P (paraffin).
  • Pitter, K.L. et al. (2011) PLoS One 6, e14545. Applications: IHC-P (paraffin).
  • Yanagawa, N. et al. (2012) J Thorac Oncol 7, 1513-1521. Applications: IHC-P (paraffin).
  • Van Themsche, C. et al. (2010) Mol Cancer 9, 216. Applications: Western Blotting.
  • Poliseno, L. et al. (2010) Sci Signal 3, ra29. Applications: Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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