Product Class: Other

Poly(U) Polymerase
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Item# Description List Price Web Price Qty
M0337S Poly (U) Polymerase - 60 units $128.00
$115.20
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Product Introduction

Poly(U) Polymerase catalyzes the template independent addition of UMP from UTP or AMP from ATP to the 3´ end of RNA.

  • Isolated from a recombinant source
  • Supplied with 10X reaction buffer
  • Tested for the absence of DNases and RNases
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Product Information

Description

Poly(U) Polymerase catalyzes the template independent addition of UMP from UTP or AMP from ATP to the 3´ end of RNA.

Product Source

An E. coli strain that carries the cloned poly(U) polymerase gene of Schizosaccharomyces pombe Cid1.

Advantages and Features

Application Features

  • Labeling of RNA with UTP
  • Poly(U) and Poly(A) tailing of RNA for cloning
  • Studying effects of poly(U) tailing on stability and translation of RNA transferred into eukaryotic cells
  • Poly (A) tailing of 2' O-Me modified 3' ends

Properties & Usage

    Unit Definition

    One unit is defined as the amount of enzyme that incorporates 1 nmol of UMP into RNA in a 50 µl volume in 10 minutes at 37°C. 

    Reaction Conditions

    1X NEBuffer™ 2
    Supplement with 0.5 mM UTP 
    Incubate at 37°C

    1X NEBuffer™ 2
    50 mM NaCl
    10 mM Tris-HCl
    10 mM MgCl2
    1 mM DTT
    (pH 7.9 @ 25°C)

    Storage Temperature

    -20°C

    Storage Conditions

    10 mM Tris-HCl
    100 mM NaCl
    1 mM DTT
    0.1 mM EDTA
    50% Glycerol
    pH 7.5 @ 25°C

    Heat Inactivation

    65°C for 20 min

    Unit Assay Conditions

    1X NEBuffer 2, 0.5 mM 3H UTP and 5 µg yeast RNA are combined in a 50 µl reaction incubated at 37°C for 10 minutes.

    Product Notes

    1. UTP is not included with this product.
    2. Poly(U) Polymerase in NEBuffer 2 will incorporate rNMP from rNTP into RNA. Tailing length of poly(U) varies with UTP. Poly(U) Polymerase is highly processive under low primer concentrations (<100 pmol).

    References

    1. Wickens, M. and Kwak, J.E. (2008). Science. 319, 1344.
    2. Kwak, J.E. and Wickens, M. (2008). RNA. 13, 860.
    3. Rissland, O.O.S., Mikulasova, A. and Norbury, C.J. (2007). Molecular and Cell Biology. 27, 3612.

    Protocols, Manuals & Usage

    Protocols

    1. A Typical Tailing Reaction (M0337)

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)

    Tools & Resources

    Selection Charts

    • RNA Polymerase Selection Chart

    FAQs & Troubleshooting

    FAQs

    1. What is the molecular weight of Poly(U) Polymerase?
    2. What type of labeled-UTPs will the Poly(U) Polymerase incorporate?
    3. Will Poly(U) Polymerase incorporate GTPs onto the 3’ end of an RNA?
    4. What Poly(U) length can be expected to be incorporated by Poly(U) Polymerase?
    5. Can Poly(U) Polymerase incorporate GTPs onto an RNA that contains chemically modified or 2-O-methyl groups on its 3’ end?
    6. How can the Poly(U) Polymerase be inactivated without heating up the reaction?
    7. Does the E. coli Poly(U) Polymerase work in the M-MuLV reverse transcriptase buffer?
    8. Can Poly(U) Polymerase be used to add a poly U tail to ssDNA?

    Troubleshooting

    Tech Tips

    We strongly recommend wearing gloves, using nuclease-free tubes and reagents, and thoroughly cleaning pipettes and bench surfaces to avoid RNase contamination.

    Quality, Safety & Legal

    Quality Assurance Statement

    Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    Specifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    Certificate Of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Safety DataSheets

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Legal and Disclaimers

    This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

    While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

    For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

    This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.