Product Class: Other

E. coli RNA Polymerase, Core Enzyme
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Item# Description List Price Web Price Qty
M0550S E.coli RNA Polymerase, Core Enzyme - 100 units $255.00
$229.50
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Product Introduction

E. coli RNA Polymerase, Core Enzyme consists of 5 subunits designated α, α, β´, β, and ω.

  • Free of sigma factor and does not recognize any specific bacterial or phage DNA promoters.
  • Retains the ability to transcribe RNA from nonspecific initiation sequences.
  • Addition of sigma factors will allow the enzyme to initiate RNA synthesis from specific bacterial and phage promoters.
  • The core enzyme has a molecular weight of approximately 400 kDa.
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Product Information

Description

Product Source

E. coli RNA Polymerase, Core Enzyme is isolated from E. coli strain BL21.

Advantages and Features

Application Features

  • RNA synthesis from E. coli promoter
  • Transcription initiation studies
  • In vitro translation with PURExpress

Properties & Usage

    Unit Definition

    One unit is defined as the amount of enzyme required to incorporate 1 nmol NTP into RNA in 10 minutes at 37°C.

    Storage Temperature

    -20°C

    Storage Conditions

    20 mM Tris-HCl
    100 mM NaCl
    0.1 mM EDTA
    1 mM DTT
    50% Glycerol
    pH 7.5 @ 25°C

    Unit Assay Conditions

    1X E. coli RNA Polymerase Reaction Buffer, supplemented with 0.5 mM of each NTP, sigma factor 70 and 1 μg T7 phage DNA in 50 μl.

    Protocols, Manuals & Usage

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)

    FAQs & Troubleshooting

    FAQs

    1. What is the difference between the E.coli RNA Polymerase, Core Enzyme and Holoenzyme?
    2. What are the major functions of these subunits?
    3. Can the Core and Holoenzyme be used in PURExpress?
    4. What are the other sigma factors in E.coli?

    Troubleshooting

    Quality, Safety & Legal

    Quality Assurance Statement

    Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    Specifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    Certificate Of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Safety DataSheets

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Legal and Disclaimers

    This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

    While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

    For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

    This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

    Trademarks

    New England Biolabs® and PUREXPRESS® are registered trademarks of New England Biolabs, Inc.
    TRITON X-100™ is a trademark of The Dow Chemical Company.