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Product Pathways - Angiogenesis

CTGF (E2W5M) Rabbit mAb #10095

Item# Description List Price Web Price Qty
10095S CTGF (E2W5M) Rabbit mAb - 100 µl $364.00
$327.60
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human Endogenous 16-20, 35 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting

Homology

Species predicted to react based on 100% sequence homology: Chicken, Bovine.

Protocols

Specificity / Sensitivity

CTGF (E2W5M) Rabbit mAb recognizes endogenous levels of total CTGF protein. This antibody recognizes both full-length CTGF, and cleaved C-terminal fragments of CTGF, which vary in length (16-20 kDa) depending upon cleavage context.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro265 of human CTGF protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CTGF (E2W5M) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

Connective tissue growth factor (CTGF, CCN2) belongs to the CCN (CYR61, CTGF, NOV) family of secreted extracellular matrix (ECM) proteins (1). Members of this family contain four conserved cysteine-rich domains, and interact in the ECM with a diverse array of cell surface receptors, including integrins and heparin-sulfate proteoglycans (2). These interactions regulate a multitude of cellular and tissue functions, including adhesion, proliferation, migration, differentiation, senescence, angiogenesis, inflammation, and wound repair (1, 3-5). The CTGF gene is a transcriptional target of both YAP/TAZ and TGFβ-SMAD signaling pathways (6,7), and aberrant regulation of CTGF expression is strongly associated with pathological conditions, notably cancer and fibrosis (8, 9).

  1. Chen, C.C. and Lau, L.F. (2009) Int J Biochem Cell Biol 41, 771-83.
  2. Gao, R. and Brigstock, D.R. (2004) J Biol Chem 279, 8848-55.
  3. Hall-Glenn, F. and Lyons, K.M. (2011) Cell Mol Life Sci 68, 3209-17.
  4. Hashimoto, G. et al. (2002) J Biol Chem 277, 36288-95.
  5. Ivkovic, S. et al. (2003) Development 130, 2779-91.
  6. Di Benedetto, A. et al. (2016) Oncotarget 7, 43188-43198.
  7. Liu, H. et al. (2015) Cell Biochem Biophys 73, 775-81.
  8. Brigstock, D.R. (2010) J Cell Commun Signal 4, 1-4.
  9. Jun, J.I. and Lau, L.F. (2011) Nat Rev Drug Discov 10, 945-63.

Application References

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