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Product Pathways - Innate Immunity

Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb (Alexa Fluor® 647 Conjugate) #10327

Item# Description List Price Web Price Qty
10327S Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb (Alexa Fluor® 647 Conjugate) - 100 µl $468.00
$421.20
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10327T Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb (Alexa Fluor® 647 Conjugate) - 20 µl $184.00
$165.60
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Companion Products

Item# Description List Price Web Price Qty
29047S Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb - 100 µl $433.00
$389.70
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Application Dilution Species-Reactivity Sensitivity Isotype
F Human, Mouse, Rat Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot using the unconjugated antibody.

Applications Key: F=Flow Cytometry

Protocols

Specificity / Sensitivity

Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of IRF-3 protein only when phosphorylated at Ser396.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser396 of human IRF-3 protein.

Flow Cytometry

Flow Cytometry

Flow Cytometric analysis of HT-29 cells, untransfected (blue) or transfected with Poly (I:C) (green) using Phospho-IRF-3 (Ser396) (D601M) Rabbit mAb (Alexa Fluor® 647 Conjugate).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb #29047.

Background

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).

  1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
  2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.

Application References

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