New England Biolabs Canada
 

Product Pathways - Cell Cycle / Checkpoint

Mre11 Blocking Peptide #1035

Item# Description List Price Web Price Qty
1035S Mre11 Blocking Peptide - 100 µg $458.00
$412.20
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
4847S Mre11 (31H4) Rabbit mAb - 100 µl $372.00
$334.80
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4847T Mre11 (31H4) Rabbit mAb - 20 µl $159.00
$143.10
ADD TO CART
4895S Mre11 Antibody - 100 µl $372.00
$334.80
ADD TO CART
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This product is assembled upon order. Please allow up to three weeks for your product to be processed.

Description

This peptide is used specifically to block Mre11 Antibody #4895 and Mre11 (31H4) Rabbit mAb #4847 reactivity.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Mre11 Antibody #4895 and Mre11 (31H4) Rabbit mAb #4847 by dot blot.

Directions For Use

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.

Background

Mre11, originally described in genetic screens from the yeast Saccharomyces cerevisiae in which mutants were defective in meiotic recombination (1), is a central part of a multisubunit nuclease composed of Mre11, Rad50 and Nbs1 (MRN) (2,3). The MRN complex plays a critical role in sensing, processing and repairing DNA double strand breaks. Defects lead to genomic instability, telomere shortening, aberrant meiosis and hypersensitivity to DNA damage (4). Hypomorphic mutations of Mre11 are found in ataxia-telangiectasia-like disease (ATLD), with phenotypes similar to mutations in ATM that cause ataxia-telangiectasia (A-T), including a predisposition to malignancy in humans (5). Cellular consequences of ATLD include chromosomal instability and defects in the intra-S phase and G2/M checkpoints in response to DNA damage. The MRN complex may directly activate the ATM checkpoint kinase at DNA breaks (6).

  1. Ajimura, M. et al. (1993) Genetics 133, 51-66.
  2. D'Amours, D. and Jackson, S.P. (2002) Nat Rev Mol Cell Biol 3, 317-27.
  3. van den Bosch, M. et al. (2003) EMBO Rep 4, 844-9.
  4. Theunissen, J.W. et al. (2003) Mol Cell 12, 1511-23.
  5. Stewart, G.S. et al. (1999) Cell 99, 577-87.
  6. Carson, C.T. et al. (2003) EMBO J 22, 6610-20.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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