New England Biolabs Canada
 

Product Pathways - Cell Cycle / Checkpoint

Cyclin D1 Blocking Peptide #1044

Item# Description List Price Web Price Qty
1044S Cyclin D1 Blocking Peptide - 100 µg $458.00
$412.20
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
2922S Cyclin D1 Antibody - 100 µl $372.00
$334.80
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2978S Cyclin D1 (92G2) Rabbit mAb - 100 µl $372.00
$334.80
ADD TO CART
2978T Cyclin D1 (92G2) Rabbit mAb - 20 µl $159.00
$99.00
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This product is assembled upon order. Please allow up to three weeks for your product to be processed.

Description

This peptide is used to block Cyclin D1 (92G2) Rabbit mAb #2978 reactivity.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Cyclin D1 (92G2) Rabbit mAb #2978 by dot blot.

Directions For Use

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.

Background

Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).

  1. Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.
  2. Sherr, C.J. (1996) Science 274, 1672-7.
  3. Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25.
  4. Diehl, J.A. et al. (1997) Genes Dev 11, 957-72.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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