New England Biolabs Canada
 

Product Pathways - Protein Translation

Phospho-4E-BP1 (Thr37/46) Blocking Peptide #1052

Item# Description List Price Web Price Qty
1052S Phospho-4E-BP1 (Thr37/46) Blocking Peptide - 100 µg $472.00
$424.80
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This product is assembled upon order. Please allow up to three weeks for your product to be processed.

Description

This peptide is used to block Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb #2855 reactivity.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide reacts with Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb #2855 signal in dot blot.

Directions For Use

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.

Background

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

  1. Pause, A. et al. (1994) Nature 371, 762-7.
  2. Brunn, G.J. et al. (1997) Science 277, 99-101.
  3. Gingras, A.C. et al. (1998) Genes Dev 12, 502-13.
  4. Fadden, P. et al. (1997) J Biol Chem 272, 10240-7.
  5. Gingras, A.C. et al. (1999) Genes Dev 13, 1422-37.

Application References

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