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Product Pathways - Cell Cycle / Checkpoint

p21 Waf1/Cip1 Blocking Peptide #1055

Item# Description List Price Web Price Qty
1055S p21 Waf1/Cip1 Blocking Peptide - 100 µg $458.00
$412.20
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
2947S p21 Waf1/Cip1 (12D1) Rabbit mAb - 100 µl $372.00
$334.80
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2947T p21 Waf1/Cip1 (12D1) Rabbit mAb - 20 µl $159.00
$143.10
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This product is assembled upon order. Please allow up to three weeks for your product to be processed.

Description

This peptide is used to block p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 reactivity in dot blot protocols.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide reacts with p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 signal in dot blot.

Directions For Use

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.

Background

The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).

  1. Pestell, R.G. et al. (1999) Endocrine Rev. 20, 501-34.
  2. Cheng, J. et al. (1999) EMBO J. 18, 1571-83.
  3. Flores-Rozas, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 8655-9.
  4. Wang, Y. and Prives, C. (1995) Nature 376, 88-91.
  5. Sheaff, R.J. et al. (2000) Cell 5, 403-10.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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