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Product Pathways - Flow Cytometry

FoxP3 (3G3) Mouse mAb (PE-Cy7® Conjugate) #10680

Item# Description List Price Web Price Qty
10680S FoxP3 (3G3) Mouse mAb (PE-Cy7® Conjugate) - 100 µg $485.00
$436.50
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Companion Products

Item# Description List Price Web Price Qty
79339S Mouse (MOPC-21) mAb IgG1 Isotype Control (PE-Cy7® Conjugate) - 500 µl $276.00
$248.40
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Application Dilution Species-Reactivity Sensitivity Isotype
F Mouse Endogenous Mouse IgG1, kappa

Applications Key: F=Flow Cytometry

Directions For Use

For optimal flow cytometry results, we recommend 0.125μg of antibody per test.

Protocols

Specificity / Sensitivity

FoxP3 (3G3) Mouse mAb (PE-Cy7® Conjugate) recognizes endogenous levels of total FoxP3 protein. This antibody detects an epitope within the intracellular domain.

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of mouse splenocytes using FoxP3 (3G3) Mouse mAb (PE-Cy7® Conjugate) (right) and co-stained with CD4-APC, compared to a concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control (PE-Cy7® Conjugate) #79339 (left).

Description

This Cell Signaling Technology antibody is conjugated to PE-Cy7® and tested in-house for direct flow cytometric analysis in mouse cells.

Background

Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 - FoxP4, which are broadly expressed and play important roles in organ development, immune response and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5).

FoxP3 is crucial for the development of T cells with regulatory properties (Treg) (6). Mutations in FoxP3 are associated with immune dysregulation, polyendocrinopathy, enteropathy, and X-linked syndrome (IPEX) (7), while overexpression in mice causes severe immunodeficiency (8). Research studies have shown that FoxP3 functions as a tumor suppressor in several types of cancer (9-11).

  1. Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
  2. Shu, W. et al. (2001) J Biol Chem 276, 27488-97.
  3. Lu, M.M. et al. (2002) Gene Expr Patterns 2, 223-8.
  4. Koon, H.B. et al. (2007) Expert Opin Ther Targets 11, 955-65.
  5. Li, S. et al. (2004) Mol Cell Biol 24, 809-22.
  6. Ochs, H.D. et al. (2007) Immunol Res 38, 112-21.
  7. Bennett, C.L. et al. (2001) Nat Genet 27, 20-1.
  8. Kasprowicz, D.J. et al. (2003) J Immunol 171, 1216-23.
  9. Zuo, T. et al. (2007) Cell 129, 1275-86.
  10. Zuo, T. et al. (2007) J Clin Invest 117, 3765-73.
  11. Wang, L. et al. (2009) Cancer Cell 16, 336-46.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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